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The Bacillus subtilis NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion.
Ogura, Mitsuo; Tsukahara, Kensuke; Hayashi, Kentaro; Tanaka, Teruo.
Afiliación
  • Ogura M; Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan.
  • Tsukahara K; Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan.
  • Hayashi K; Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan.
  • Tanaka T; Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido-Shimizu, Shizuoka 424-8610, Japan.
Microbiology (Reading) ; 153(Pt 3): 667-675, 2007 Mar.
Article en En | MEDLINE | ID: mdl-17322186
A previous microarray analysis suggested that multicopy yccH, encoding a function-unknown response regulator, enhances expression of natAB, which encodes a two-gene ATP-binding cassette transporter involved in the extrusion of sodium ions. The two-component regulatory system YccG-YccH was therefore renamed NatK-NatR. Here, this observation was confirmed by a lacZ fusion analysis using a strain carrying natA-lacZ. Further, in both natK and natR mutants, natA-lacZ expression was completely abolished, indicating that the NatK-NatR system positively regulates the expression of natAB. In a gel retardation analysis, NatR bound to the natA promoter region. Using purified His-tagged NatR, DNase I footprinting analysis of the natA promoter region suggested that a direct repeat of [TTCA(G)CGACA], separated by a 12 bp space, would be recognized by NatR. Deleted and mutagenized promoter regions of natA were analysed using a lacZ fusion, and it was confirmed that the direct repeat is critical for natA activation by NatR.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sodio / Bacillus subtilis / Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Transportadoras de Casetes de Unión a ATP Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2007 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sodio / Bacillus subtilis / Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Transportadoras de Casetes de Unión a ATP Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2007 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido