Possible peptide chain termination mutants in thymide kinase gene of a mammalian virus, herpes simplex virus.
Proc Natl Acad Sci U S A
; 72(10): 4081-4, 1975 Oct.
Article
en En
| MEDLINE
| ID: mdl-172894
Mutations in the viral gene coding for the thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.75) induced by herpes simplex virus have been obtained by selection of virus resistant to bromodeoxyuridine when grown in thymidine-kinase-deficient LMTK- mouse cells. Proteins labeled after infection of Vero (monkey) cells with herpes simplex virus were analyzed by gel electrophoresis and one protein of about 40,000 daltons was consistently altered in a number of thymidine-kinase-deficient mutants. Many viral mutants lacked this peptide and one class of these mutants induced the synthesis of new shorter peptides. Revertant virus could be selected which simultaneously regained the ability to induce thymidine kinase activity, regained the intact thymidine kinase peptide, and lost the ability to synthesize the shorter peptide fragment. These mutants comprise a class of animal virus mutants which have the properties expected of peptide chain termination mutants.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Terminación de la Cadena Péptídica Traduccional
/
Timidina Quinasa
/
Transcripción Genética
/
Simplexvirus
/
Genes
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
1975
Tipo del documento:
Article
Pais de publicación:
Estados Unidos