Mannan-modified adenovirus as a vaccine to induce antitumor immunity.

Ding, Z-Y; Wu, Y; Luo, Y; Su, J-M; Li, Q; Zhang, X-W; Liu, J-Y; He, Q-M; Yang, L; Tian, L; Zhao, X; Deng, H-X; Wen, Y-J; Li, J; Kang, B; Wei, Y-Q

Ding, Z-Y; Wu, Y; Luo, Y; Su, J-M; Li, Q; Zhang, X-W; Liu, J-Y; He, Q-M; Yang, L; Tian, L; Zhao, X; Deng, H-X; Wen, Y-J; Li, J; Kang, B; Wei, Y-Q.

Afiliación

Ding ZY; Department of Oncology, National Key Laboratory of Biotherapy, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Sichuan, The People's Republic of China.

Gene Ther ; 14(8): 657-63, 2007 Apr.

Article en En | MEDLINE | ID: mdl-17287861

RESUMEN

Tumor vaccine is a useful strategy for cancer therapy. However, priming of the immune system requires the relevant antigen to be presented by antigen-presenting cells (APCs). Here, we employed telomerase reverse transcriptase as a model antigen to explore the feasibility of using mannan-modified adenovirus as a tumor vaccine. We found that tumor immunogene therapy with the vaccine was effective at protective antitumor immunity in mice. The antigen-specific cytotoxic T lymphocytes were found in in vitro cytotoxicity assay. The elevation of the killing activity could be abrogated by anti-CD8 or anti-major histocompatibility complex-I antibodies. Adoptive transfer of purified CD8+ cells, and CD4+ cells to a less extent, was effective at antitumor activity. In vivo antitumor activity could be abrogated by depleting CD4+ T lymphocytes. A possible explanation for the antitumor effects may be the antigen was transferred to APCs in the presence of mannan. These observations provide insights into the design of novel vaccine strategies and might be important for the future application of antigens identified in other diseases.

Asunto(s)

Adenoviridae/genética; Antígenos de Neoplasias/inmunología; Vacunas contra el Cáncer/administración & dosificación; Terapia Genética/métodos; Mananos/genética; Melanoma Experimental/terapia; Traslado Adoptivo; Animales; Presentación de Antígeno; Linfocitos T CD4-Positivos/inmunología; Linfocitos T CD8-positivos/inmunología; Vacunas contra el Cáncer/genética; Células Dendríticas/inmunología; Células Dendríticas/virología; Ingeniería Genética; Depleción Linfocítica; Mananos/metabolismo; Melanoma Experimental/inmunología; Ratones; Ratones Endogámicos BALB C

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Melanoma Experimental / Terapia Genética / Adenoviridae / Vacunas contra el Cáncer / Mananos / Antígenos de Neoplasias Límite: Animals Idioma: En Revista: Gene Ther Asunto de la revista: GENETICA MEDICA / TERAPEUTICA Año: 2007 Tipo del documento: Article Pais de publicación: Reino Unido

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