Adhesion and proliferation of fibroblasts on cluster-assembled nanostructured carbon films: the role of surface morphology.

Lenardi, C; Perego, C; Cassina, V; Podestà, A; D'Amico, A; Gualandris, D; Vinati, S; Fiorentini, F; Bongiorno, G; Piserj, P; Vellea Sacchi, F; Milani, P

Lenardi, C; Perego, C; Cassina, V; Podestà, A; D'Amico, A; Gualandris, D; Vinati, S; Fiorentini, F; Bongiorno, G; Piserj, P; Vellea Sacchi, F; Milani, P.

Afiliación

Lenardi C; Istituto di Fisiologia Generale e Chimica Biologica Università di Milano, Via Trentacoste 2, 1-20134 Milano, Italy.

J Nanosci Nanotechnol ; 6(12): 3718-30, 2006 Dec.

Article en En | MEDLINE | ID: mdl-17256321

RESUMEN

We have investigated the influence on adhesion and proliferation of NIH 3T3 fibroblasts of the surface morphology of cluster assembled carbon films deposited by Supersonic Cluster Beam Deposition. Nanostructured carbon films exhibit a multi-scale morphology, which resembles the surface structure of the extracellular matrix, and possess a high specific area, while being relatively smooth at all scales. Correlations between measured morphological parameters and adaptive cell response have been brought out. High specific area and smoothness appear to conceivably favour both the early attachment of plated cells and the long-term survival of adherent cells. Moreover, nano-structured carbon films affect the cells morphology as well as the extension and the number of the focal contacts.

Asunto(s)

Carbono/química; Técnicas de Cultivo de Célula/métodos; Fibroblastos/citología; Fibroblastos/fisiología; Nanoestructuras/química; Nanotecnología/métodos; Ingeniería de Tejidos/métodos; Animales; Adhesión Celular; Proliferación Celular; Cristalización/métodos; Sustancias Macromoleculares/química; Ensayo de Materiales; Membranas Artificiales; Ratones; Conformación Molecular; Células 3T3 NIH; Nanoestructuras/ultraestructura; Tamaño de la Partícula; Propiedades de Superficie

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbono / Técnicas de Cultivo de Célula / Ingeniería de Tejidos / Nanotecnología / Nanoestructuras / Fibroblastos Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: J Nanosci Nanotechnol Año: 2006 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

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