Evolutionary trends of neurofilament proteins in fish.
Comp Biochem Physiol B
; 100(4): 733-40, 1991.
Article
en En
| MEDLINE
| ID: mdl-1723670
1. Neurofilament complement was studied in an early chordate (Ciona intestinalis) and six fish species by immunoblot with antisera specific for each of the three mammalian NF subunits. 2. The anti-NF-H and anti-NF-M antisera were characterized as strictly specific for phosphorylated epitopes located in the carboxyterminal domain. 3. The NF-L subunit is absent in primitive chordates and appears first in fish; it can be identified on the basis of its apparent mol. wt, its reactivity with the anti-IFA antibody and with polyclonal antibodies raised to the NF-L subunit of mammals. 4. Primitive chordate neurofilaments are constituted by a single polypeptide of ca 160,000 mol. wt exhibiting only M-type phosphorylation-dependent epitopes. 5. Primitive fish (Acipenser transmontanus, Salmo gairdneri, Scorpaena porcus, Serranus scriba) possess only a single high mol. wt NF subunit reacting with both anti-NF-H and anti-NF-M antiserum while more recent species (Mugil saliens, Perca fluviatilis) possess two high mol. wt NF subunits which are immunologically distinct as to their phosphorylation structures. 6. The existence in some fish species of two high mol. wt NF polypeptides suggests that the process of gene duplication and diversification supposed to have given rise to the two high mol. wt NF subunits of mammals and birds has occurred repeatedly in vertebrate evolution, and may be regarded as a case of convergent evolution.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Neurofilamentos
/
Evolución Biológica
/
Peces
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol B
Año:
1991
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido