Adrenomedullin gene expression and peptide levels in the heart and blood vessels of streptozotocin-diabetic rats.
Horm Metab Res
; 39(1): 3-8, 2007 Jan.
Article
en En
| MEDLINE
| ID: mdl-17226106
The aim of the present study was to assess the changes in gene expression and peptide adrenomedullin (AM) levels in cardiovascular and other tissues in the streptozotocin-diabetic rats. For this purpose, diabetes was induced by intraperitoneal injection of streptozotocin (STZ, 65 mg/Kg body weight). Half of the diabetic rats were subcutaneously injected with insulin in the afternoon (4 units/day) one week after STZ injection until the day before killing. Control rats received only saline injection. AM mRNA was determined in cardiovascular and other tissues of streptozotocin-diabetic rats using solution-hybridization-RNase protection assay. Circulating AM and peptide AM in cardiovascular and other tissues were estimated using a specific radioimmunoassay. There were increases in preproAM mRNA levels in the left and right ventricles and in the thoracic aorta in both the 2-week and 4-week diabetic rats, but not in the two atria, the mesenteric artery and the lung. In the 2-week diabetic rats, there were decreases in AM contents in the two atria and the lung but an increase in the thoracic aorta. In the 4-week diabetic rats, there were bigger decreases in the atria and also a decrease in the left ventricle. The plasma AM levels were not changed but there was an increase in the excretion of AM in the urine. Our results suggest a possible increase in AM release in the heart and the thoracic aorta in the 2-week and 4-week diabetic rats.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vasos Sanguíneos
/
Diabetes Mellitus Experimental
/
Adrenomedulina
/
Miocardio
Límite:
Animals
Idioma:
En
Revista:
Horm Metab Res
Año:
2007
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Alemania