Enhanced ceramide-induced apoptosis in ceramide kinase overexpressing cells.
Biochem Biophys Res Commun
; 354(1): 309-14, 2007 Mar 02.
Article
en En
| MEDLINE
| ID: mdl-17222802
We evaluated how increased levels of ceramide kinase (CerK) would impact the growth of COS-1 fibroblasts and RBL-2H3 basophils. The low CerK activity in these cells was strongly up-regulated upon recombinant expression of CerK. CerK-overexpressing COS-1 cells depended on higher concentrations of serum for their growth and displayed many filipodia. The two CerK-overexpressing cell lines were more sensitive to C2-ceramide-mediated apoptosis, and this correlated with the production of C2-ceramide-1-phosphate by CerK. This study indicates that ceramide kinase may participate in the control of cell growth, and establishes a novel assay that will be valuable for testing ceramide kinase inhibitors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ceramidas
/
Apoptosis
/
Fosfotransferasas (Aceptor de Grupo Alcohol)
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2007
Tipo del documento:
Article
País de afiliación:
Austria
Pais de publicación:
Estados Unidos