Asef is a Cdc42-specific guanine nucleotide exchange factor.
Biol Chem
; 388(1): 67-71, 2007 Jan.
Article
en En
| MEDLINE
| ID: mdl-17214551
Asef is a member of the Dbl-family of guanine nucleotide exchange factors (GEFs) with a proposed specificity for the small GTPase Rac1. Here we investigated the specificity and regulation of Asef by measuring its GEF activity in vitro and observed hardly any activity towards Rac1, Rac2 and Rac3, or RhoA and TC10. In contrast, various purified Asef protein fragments catalyzed the nucleotide exchange reaction of Cdc42. The Cdc42GEF activity of the Dbl homology (DH) domain of Asef was significantly higher in the presence of the pleckstrin homology (PH) domain. Our data strongly suggest that Asef is a canonical Cdc42GEF, which employs its PH domain to efficiently stabilize its autoinhibited state, but also to facilitate nucleotide exchange activity of the DH domain after its activation by upstream signals.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína de Unión al GTP cdc42
/
Factores de Intercambio de Guanina Nucleótido
Idioma:
En
Revista:
Biol Chem
Asunto de la revista:
BIOQUIMICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Alemania