Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro.

Zhang, Zhen-Xing; Guan, Li-Xue; Zhang, Kun; Wang, Shouxian; Cao, Pei-Cheng; Wang, Yong-He; Wang, Zhe; Dai, Long-Jun

Zhang, Zhen-Xing; Guan, Li-Xue; Zhang, Kun; Wang, Shouxian; Cao, Pei-Cheng; Wang, Yong-He; Wang, Zhe; Dai, Long-Jun.

Afiliación

Zhang ZX; Department of Neurosurgery, Weifang People's Affiliated Hospital, Weifang Medical College, Weifang 261041, PR China.

Cell Biol Int ; 31(6): 645-8, 2007 Jun.

Article en En | MEDLINE | ID: mdl-17207646

RESUMEN

This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.

Asunto(s)

Células de la Médula Ósea/citología; Análisis Citogenético/métodos; Células Madre Mesenquimatosas/citología; Adulto; Células Cultivadas; Preescolar; Bandeo Cromosómico; Cromosomas Humanos/genética; Femenino; Humanos; Cariotipificación; Masculino; Metafase; Persona de Mediana Edad

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Análisis Citogenético / Células Madre Mesenquimatosas Límite: Adult / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Biol Int Año: 2007 Tipo del documento: Article Pais de publicación: Reino Unido

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