F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses.
Nat Biotechnol
; 25(1): 91-9, 2007 Jan.
Article
en En
| MEDLINE
| ID: mdl-17187059
A useful approach for exploring gene function involves generating mutant mice from genetically modified embryonic stem (ES) cells. Recent advances in genetic engineering of ES cells have shifted the bottleneck in this process to the generation of mice. Conventional injections of ES cells into blastocyst hosts produce F0 generation chimeras that are only partially derived from ES cells, requiring additional breeding to obtain mutant mice that can be phenotyped. The tetraploid complementation approach directly yields mice that are almost entirely derived from ES cells, but it is inefficient, works only with certain hybrid ES cell lines and suffers from nonspecific lethality and abnormalities, complicating phenotypic analyses. Here we show that laser-assisted injection of either inbred or hybrid ES cells into eight cell-stage embryos efficiently yields F0 generation mice that are fully ES cell-derived and healthy, exhibit 100% germline transmission and allow immediate phenotypic analysis, greatly accelerating gene function assignment.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ratones Transgénicos
/
Marcación de Gen
/
Trasplante de Células Madre
/
Células Madre Embrionarias
/
Terapia por Láser
/
Microinyecciones
Límite:
Animals
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos