[Quality analysis and evaluation of Rhizoma Coptidis under different cultivation conditions].
Yao Xue Xue Bao
; 41(10): 1010-4, 2006 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-17184122
AIM: To develop methods for the fingerprint analysis of Rhizoma Coptidis and the determination of berberine, palmatine and jatrorrhizine in Rhizoma Coptidis, and analyze the contents of these three alkaloids in Rhizoma Coptidis under different cultivation conditions, from different areas and processed with different methods. METHODS: Two methods (HPLC-UV and HPLC-MS) have been developed and used in fingerprint analysis of Rhizoma Coptidis. An HPLC method was used to determine the contents of three alkaloids. RESULTS: With HPLC-MS techniques, seven major chromatographic peaks in the fingerprint analysis of Rhizoma Coptidis were identified by their MS spectra and compared with the reference standards. In different cultivation conditions, shading conditions and growing ages have obvious influence on the contents of three alkaloids in Rhizoma Coptidis, while planting density was not the major factor that influenced the contents of three alkaloids. The contents of three alkaloids of Coptidis samples were almost higher than those of Coptidis reference material. For Coptidis samples from different cultivation area, the contents of these three alkaloids were different greatly. For Coptidis samples processed with different methods, the contents of three alkaloids were not influenced obviously by processing methods. CONCLUSION: The results showed that the ecology cultivation method to replace the traditional shading method was feasible and provided the theoretical foundation for scientifically processing Rhizoma Coptidis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Berberina
/
Alcaloides de Berberina
/
Coptis
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Año:
2006
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China