A comparative study of the ribosomal RNA operons of Streptomyces coelicolor A3(2) and sequence analysis of rrnA.
Nucleic Acids Res
; 19(16): 4399-403, 1991 Aug 25.
Article
en En
| MEDLINE
| ID: mdl-1715981
S. coelicolor A3(2) contains six ribosomal RNA operons. Here we describe the cloning of rrnA, rrnC and rrnE, thereby completing the cloning of all operons. Southern hybridisation of genomic DNA with a heterologous probe from the E.coli rrnB 16S rRNA gene showed differences in hybridisation among the six rRNA operon-containing bands. The nucleotide sequence of the 16S rRNA gene and the upstream region of rrnA was determined and compared with the corresponding sequence of rrnD, showing that the 16S rRNA genes are 99% identical. Substantial differences were found, however, in the upstream regions corresponding to the P1 and P2 promoters of rrnD. Southern analysis showed that some of the other rRNA operons of S.coelicolor A3(2) also differed in this part of the upstream region.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Operón
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Streptomyces
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ARN Ribosómico 5S
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ARN Ribosómico 16S
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ARN Ribosómico 23S
Idioma:
En
Revista:
Nucleic Acids Res
Año:
1991
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Reino Unido