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Frizzled/PCP-dependent asymmetric neuralized expression determines R3/R4 fates in the Drosophila eye.
del Alamo, David; Mlodzik, Marek.
Afiliación
  • del Alamo D; Department of Molecular, Cell, and Developmental Biology, Mount Sinai School of Medicine, New York, New York 10029, USA.
Dev Cell ; 11(6): 887-94, 2006 Dec.
Article en En | MEDLINE | ID: mdl-17141162
Planar cell polarity (PCP) is a common feature in many epithelia, reflected in cellular organization within the plane of an epithelium. In the Drosophila eye, Frizzled (Fz)/PCP signaling induces cell-fate specification of the R3/R4 photoreceptors through regulation of Notch activation in R4. Except for Dl upregulation in R3, the mechanism of how Fz/PCP signaling regulates Notch in this context is not understood. We demonstrate that the E3-ubiquitin ligase Neuralized (Neur), required for Dl-N signaling, is asymmetrically expressed within the R3/R4 pair. It is required in R3, where it is also upregulated in a Fz/PCP-dependent manner. As is the case for Dl, N activity in R4 further represses neur expression, thus, reinforcing the asymmetry. We demonstrate that Neur asymmetry is instructive in correct R3/R4 specification. Our data indicate that Fz/PCP-dependent Neur expression in R3 ensures the proper directionality of Dl-N signaling during R3/R4 specification.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polaridad Celular / Receptores de Fenciclidina / Proteínas de Drosophila / Ubiquitina-Proteína Ligasas / Receptores Acoplados a Proteínas G / Drosophila melanogaster / Receptores Frizzled / Ojo Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polaridad Celular / Receptores de Fenciclidina / Proteínas de Drosophila / Ubiquitina-Proteína Ligasas / Receptores Acoplados a Proteínas G / Drosophila melanogaster / Receptores Frizzled / Ojo Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos