[The protective effects of ginsenoside RG1 and Rb1 against damage of HaCaT cells by ultraviolet B].
Yao Xue Xue Bao
; 41(9): 905-8, 2006 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-17111842
AIM: To investigate the survival rate and the level of HaCaT cells damage with ultraviolet B (UVB) radiation at various doses, and observe the protective effects of ginsenoside Rg1 and Rb1 in vitro. METHODS: MTT assay was employed to analyze the cell survival rate after UVB radiation of 30, 60, 90 and 120 mJ x cm(-2). The damage of nucleolus and the protective effects of ginsenoside Rg1 and Rb1 were scanned by Hoechst 33258 staining and single cell gel electrophoresis assay (SCGE). RESULTS: It was found that the cell survival rate decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ x cm(-2) to 120 mJ x cm(-2). At the dose of 20 microg x mL(1-), obvious protective effect of ginsenoside Rg1 and Rb1 can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage. CONCLUSION: UVB radiation inhibits HaCaT human keratinocytes growth and ginsenoside Rg1 and Rb1 can relief the damage.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Queratinocitos
/
Apoptosis
/
Ginsenósidos
Límite:
Humans
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Año:
2006
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China