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Detection of an Alu insertion in the POMT1 gene from three French Walker Warburg syndrome families.
Bouchet, C; Vuillaumier-Barrot, S; Gonzales, M; Boukari, S; Bizec, C Le; Fallet, C; Delezoide, A-L; Moirot, H; Laquerriere, A; Encha-Razavi, F; Durand, G; Seta, N.
Afiliación
  • Bouchet C; APHP, Bichat Claude-Bernard Hospital, Biochimie métabolique et cellulaire, Paris, France.
Mol Genet Metab ; 90(1): 93-6, 2007 Jan.
Article en En | MEDLINE | ID: mdl-17079174
Walker Warburg syndrome (WWS) is the most severe of a group of multiple congenital disorders known as lissencephaly type II ( LIS Type II) associated with congenital muscular dystrophy and eye abnormalities. The POMT1 gene is the most frequently affected found in 20% of patients with WWS. We describe five fetuses with WWS in three non-related families carrying a same mutation in the POMT1 gene. All fetuses presented with tetra ventricular hydrocephaly, and arachnoidal neuroglial ectopia and cortical dysplasia characteristic of LIS type II. We performed sequencing of the POMT1 gene on fetal DNA. The five fetuses were found to share an insertion of an inversed Alu repeated DNA element within exon 3 of the POMT1 gene, all at the heterozygous state except one at the homozygous state. This mutation was associated with a common transition c.2203 C > T (p.Arg735Cys) in exon 20 on the same allele and similar intragenic haplotype, suggesting that the three families could be related or indicating a possible founder effect in France. Insertions of Alu sequences, which are rarely found in coding regions, have occasionally been reported to cause other genetic diseases. However, this is the first report of a retrotransposon insertion in the POMT1 gene associated with WWS.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anomalías Múltiples / Elementos Alu Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy País/Región como asunto: Europa Idioma: En Revista: Mol Genet Metab Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA / METABOLISMO Año: 2007 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anomalías Múltiples / Elementos Alu Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy País/Región como asunto: Europa Idioma: En Revista: Mol Genet Metab Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA / METABOLISMO Año: 2007 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos