Characterization of a CD4-positive T-cell line derived from an athymic (nu/nu) mouse.
Cell Immunol
; 134(1): 54-64, 1991 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-1707347
We have isolated a Thy-1+, CD3+, CD4+ T-cell line from the spleen of a 12-week-old nu/nu (nude) BALB/c mouse. The cell line is clonal, and it expresses an alpha beta T-cell antigen receptor. Upon activation, these cells secrete IL-2 but not IL-4, putting them in the Th1 category. The cells can be triggered to proliferate and secrete lymphokines in the presence of irradiated syngeneic or allogeneic splenic feeder cells that express a variety of MHC haplotypes. This response is MHC class II-specific, because it can be blocked by either anti-Ia or anti-CD4 antibodies. From the response pattern of this T-cell line, we conclude that it recognizes a common determinant on class II MHC antigens. This nude mouse T-lymphocyte presumably has not undergone thymic selection. Therefore its unique specificity may reflect both the bias of T-cell antigen receptor genes for encoding receptors that recognize MHC molecules and the requirement for functional thymic epithelial cells for the efficient education of a self-MHC-restricted repertoire.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos T CD4-Positivos
/
Antígenos de Histocompatibilidad Clase II
/
Linfocitos T Colaboradores-Inductores
/
Ratones Desnudos
Límite:
Animals
Idioma:
En
Revista:
Cell Immunol
Año:
1991
Tipo del documento:
Article
Pais de publicación:
Países Bajos