Human gingival fibroblast response to electropolished NiTi surfaces.
J Biomed Mater Res A
; 80(1): 159-66, 2007 Jan.
Article
en En
| MEDLINE
| ID: mdl-17001650
In the present study the in vitro biocompatibility of electropolished NiTi sheets is investigated. The assessment of cytotoxic effects due to potential Ni leaching from metal sheets was performed in direct contact with primary human fibroblast cultures using the 5-bromo-2'-deoxyuridine cell proliferation assay and morphologic studies via light microscopy and scanning electron microscopy. To assess toxic effects related to Ni-ions release, cells cultured in the presence of increasing concentrations of Ni(2+) (NiSO(4).6H(2)O) served as positive controls. It is shown that while the addition of NiSO(4) caused severe proliferation decrease (approximately 80%) and morphologic damage at a concentration of 50 mg/L Ni(2+) no negative effects were observed in fibroblasts cultured in the presence of electropolished NiTi sheets. The results are discussed in terms of surface topography effects on the biocompatibility of NiTi shape memory alloys.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Titanio
/
Ensayo de Materiales
/
Proliferación Celular
/
Aleaciones
/
Fibroblastos
/
Encía
/
Níquel
Límite:
Humans
Idioma:
En
Revista:
J Biomed Mater Res A
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos