Genes with internal repeats require the THO complex for transcription.
Proc Natl Acad Sci U S A
; 103(39): 14423-8, 2006 Sep 26.
Article
en En
| MEDLINE
| ID: mdl-16983072
The evolutionarily conserved multisubunit THO complex, which is recruited to actively transcribed genes, is required for the efficient expression of FLO11 and other yeast genes that have long internal tandem repeats. FLO11 transcription elongation in Tho- mutants is hindered in the region of the tandem repeats, resulting in a loss of function. Moreover, the repeats become genetically unstable in Tho- mutants. A FLO11 gene without the tandem repeats is transcribed equally well in Tho+ or Tho- strains. The Tho- defect in transcription is suppressed by overexpression of topoisomerase I, suggesting that the THO complex functions to rectify aberrant structures that arise during transcription.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Secuencias Repetitivas de Ácidos Nucleicos
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Complejos Multiproteicos
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Genes Fúngicos
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos