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Plasma antioxidants in subjects before hematopoietic stem cell transplantation.
White, A C; Sousa, A M; Blumberg, J; Ryan, H F; Fanburg, B L; Kayyali, U S.
Afiliación
  • White AC; Pulmonary, Critical Care and Sleep Division, Department of Medicine, Tufts-New England Medical Center, Tupper Research Institute, Tufts University School of Medicine, Boston, MA, USA. AWhite@NESinai.org
Bone Marrow Transplant ; 38(7): 513-20, 2006 Oct.
Article en En | MEDLINE | ID: mdl-16980999
UNLABELLED: Chemo-irradiation induced oxidative damage to vascular endothelium may contribute to pulmonary complications of hematopoietic stem cell transplantation (HSCT). We measured antioxidants, markers of oxidative stress and plasma antioxidant capacity in plasma or serum from 24 subjects at day 7 before HSCT and 20 control subjects. The plasma concentration of extracellular glutathione peroxidase (GPX-3) was significantly reduced in the HSCT subjects compared with controls (HSCT: 98+/-42 microg/ml, control: 169+/-56 microg/ml, P<0.0001). The concentration of gamma-tocopherol was significantly higher in the HSCT subjects compared with controls (HSCT: 207+/-103 microg/dl; CONTROL: 98+/-52 microg/dl; P=0.0002). The plasma concentrations of protein carbonyl, nitrotyrosine, malondialdehyde, alpha-tocopherol, vitamin A, homocysteine, cysteine and cysteinylglycine did not differ between HSCT and control subjects. Plasma from HSCT subjects was as effective as control plasma in quenching menadione-induced intracellular reactive oxygen species production in human microvascular endothelial cells. In summary, subjects before HSCT have significantly reduced plasma concentrations of GPX-3, elevated plasma gamma-tocopherol yet retains the ability to quench an acute oxidative stress. These changes may play a role in chronic oxidative stress in the HSCT population.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Trasplante de Células Madre Hematopoyéticas / Glutatión Peroxidasa / Antioxidantes Tipo de estudio: Etiology_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Bone Marrow Transplant Asunto de la revista: TRANSPLANTE Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Trasplante de Células Madre Hematopoyéticas / Glutatión Peroxidasa / Antioxidantes Tipo de estudio: Etiology_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Bone Marrow Transplant Asunto de la revista: TRANSPLANTE Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido