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Peridinin-chlorophyll-protein reconstituted with chlorophyll mixtures: preparation, bulk and single molecule spectroscopy.
Brotosudarmo, T H P; Hofmann, E; Hiller, R G; Wörmke, S; Mackowski, S; Zumbusch, A; Bräuchle, C; Scheer, H.
Afiliación
  • Brotosudarmo TH; Department Biologie I, Bereich Botanik, Ludwig-Maximilians-Universität München, Menzinger Str. 67, D-80638 München, Germany.
FEBS Lett ; 580(22): 5257-62, 2006 Oct 02.
Article en En | MEDLINE | ID: mdl-16962590
Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that contain Chl a in one pigment cluster, and Chl b in the other. Binding of Chl b is stronger than that of the native pigment, Chl a. Energy transfer from Chl b to Chl a is efficient, but there are only weak interactions between the two pigments. Individual homo- and hetero-chlorophyllous complexes were investigated by single molecule spectroscopy using excitation into the peridinin absorption band and scanning of the Chl fluorescence, the latter show frequently well resolved emissions of the two pigments.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Proteínas Protozoarias / Clorofila / Eucariontes Límite: Animals Idioma: En Revista: FEBS Lett Año: 2006 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Proteínas Protozoarias / Clorofila / Eucariontes Límite: Animals Idioma: En Revista: FEBS Lett Año: 2006 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido