Evaluation of Th1-like, Th2-like and immunomodulatory cytokine mRNA expression in the skin of dogs with immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis.

Breathnach, Rory M; Fanning, Shay; Mulcahy, Grace; Bassett, Hugh F; Jones, Boyd R; Daly, Paul

Breathnach, Rory M; Fanning, Shay; Mulcahy, Grace; Bassett, Hugh F; Jones, Boyd R; Daly, Paul.

Afiliación

Breathnach RM; Department of Small Animal Clinical Studies, Faculty of Veterinary Medicine, University College Dublin, Dublin 4, Ireland. rory.breathnach@ucd.ie

Vet Dermatol ; 17(5): 313-21, 2006 Oct.

Article en En | MEDLINE | ID: mdl-16961816

RESUMEN

The term immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis (ImR-LPP) has previously been proposed to denote a subpopulation of dogs with idiopathic pododermatitis. The objective of this study was to quantify the expression of mRNA encoding Th(1)-like [interferon (IFN)-gamma, interleukin (IL)-2 and IL-12], Th(2)-like [IL-4 and IL-6] and immunomodulatory cytokines [IL-10 and transforming growth factor (TGF)-beta] in lesional ImR-LPP, nonlesional ImR-LPP and healthy control pedal skin. Gene transcripts were quantified using TaqMan real-time reverse transcriptase-polymerase chain reaction assays. The skin of dogs with ImR-LPP had significant overexpression of IL-6 mRNA (P < 0.05) and significant underexpression of IL-12 mRNA (P < 0.01) compared to healthy controls. In addition, lesional ImR-LPP skin had significantly higher levels of IL-10 transcripts compared to healthy control pedal skin (P < 0.05). Although not attaining significance (P = 0.07), a trend towards reduced TGF-beta mRNA expression in lesional ImR-LPP skin was also evident. There were no significant differences in the levels of IFN-gamma or IL-2 mRNA transcripts among the three skin sample sources. IL-4 mRNA was detected in only one lesional sample. These results suggest that the pathogenesis of ImR-LPP may be associated with a T-cell-mediated inflammatory response characterized by impaired Th(1)-like, but enhanced Th(2)-like cytokine expression.

Asunto(s)

Citocinas/genética; Dermatitis/veterinaria; Enfermedades de los Perros/inmunología; Regulación de la Expresión Génica; Piel/metabolismo; Células TH1/inmunología; Células Th2/inmunología; Animales; Citocinas/inmunología; Dermatitis/inmunología; Dermatitis/metabolismo; Enfermedades de los Perros/metabolismo; Perros; Femenino; Enfermedades del Pie/inmunología; Enfermedades del Pie/metabolismo; Enfermedades del Pie/veterinaria; Masculino; ARN Mensajero/genética; ARN Mensajero/metabolismo; Piel/inmunología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Regulación de la Expresión Génica / Citocinas / Células Th2 / Células TH1 / Dermatitis / Enfermedades de los Perros Límite: Animals Idioma: En Revista: Vet Dermatol Asunto de la revista: DERMATOLOGIA / MEDICINA VETERINARIA Año: 2006 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Reino Unido

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