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A thermally responsive biopolymer for intra-articular drug delivery.
Betre, Helawe; Liu, Wenge; Zalutsky, Michael R; Chilkoti, Ashutosh; Kraus, Virginia B; Setton, Lori A.
Afiliación
  • Betre H; Department of Biomedical Engineering, 136 Hudson Hall, Box 90821, Duke University, Durham, NC 27708, USA.
J Control Release ; 115(2): 175-82, 2006 Oct 10.
Article en En | MEDLINE | ID: mdl-16959360
Intra-articular drug delivery is the preferred standard for targeting pharmacologic treatment directly to joints to reduce undesirable side effects associated with systemic drug delivery. In this study, a biologically based drug delivery vehicle was designed for intra-articular drug delivery using elastin-like polypeptides (ELPs), a biopolymer composed of repeating pentapeptides that undergo a phase transition to form aggregates above their transition temperature. The ELP drug delivery vehicle was designed to aggregate upon intra-articular injection at 37 degrees C, and form a drug 'depot' that could slowly disaggregate and be cleared from the joint space over time. We evaluated the in vivo biodistribution and joint half-life of radiolabeled ELPs, with and without the ability to aggregate, at physiological temperatures encountered after intra-articular injection in a rat knee. Biodistribution studies revealed that the aggregating ELP had a 25-fold longer half-life in the injected joint than a similar molecular weight protein that remained soluble and did not aggregate. These results suggest that the intra-articular joint delivery of ELP-based fusion proteins may be a viable strategy for the prolonged release of disease-modifying protein drugs for osteoarthritis and other arthritides.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopolímeros / Sistemas de Liberación de Medicamentos / Inyecciones Intraarticulares Límite: Animals Idioma: En Revista: J Control Release Asunto de la revista: FARMACOLOGIA Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopolímeros / Sistemas de Liberación de Medicamentos / Inyecciones Intraarticulares Límite: Animals Idioma: En Revista: J Control Release Asunto de la revista: FARMACOLOGIA Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos