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Detection of Enterobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture.
Mullane, N R; Murray, J; Drudy, D; Prentice, N; Whyte, P; Wall, P G; Parton, A; Fanning, S.
Afiliación
  • Mullane NR; Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
Appl Environ Microbiol ; 72(9): 6325-30, 2006 Sep.
Article en En | MEDLINE | ID: mdl-16957259
Enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. Contaminated infant milk formula (IMF) has been implicated in cases of E. sakazakii meningitis. Quick and sensitive methods to detect low-level contamination sporadically present in IMF preparations would positively contribute towards risk reduction across the infant formula food chain. Here we report on the development of a simple method, combining charged separation and growth on selective agar, to detect E. sakazakii in IMF. This protocol can reliably detect 1 to 5 CFU of E. sakazakii in 500 g of IMF in less than 24 h.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Bacteriológicas / Cronobacter sakazakii / Fórmulas Infantiles / Microbiología de Alimentos Tipo de estudio: Diagnostic_studies Límite: Humans / Newborn Idioma: En Revista: Appl Environ Microbiol Año: 2006 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Bacteriológicas / Cronobacter sakazakii / Fórmulas Infantiles / Microbiología de Alimentos Tipo de estudio: Diagnostic_studies Límite: Humans / Newborn Idioma: En Revista: Appl Environ Microbiol Año: 2006 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos