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Monoclonal antibody labels both rod and cone outer segments of turtle photoreceptors.
Ohtsuka, T; Kawamata, K.
Afiliación
  • Ohtsuka T; Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Exp Eye Res ; 50(5): 483-6, 1990 May.
Article en En | MEDLINE | ID: mdl-1695575
We have studied the immunoreactivity of turtle photoreceptors to a monoclonal antibody (MAb 15-18) which binds to the external loop connecting bovine rhodopsin helices IV-V. Three chromatic types of cone photoreceptors were identified by the presence and color of oil droplets. MAb 15-18 intensely labeled the outer segments of both rods and green cones. In addition, a weak cross-reactivity was also found in the outer segments of red cones having a pale-green oil droplet, and of blue cones. Other morphological subtypes of red cones, cones with a red oil droplet and both members of double cones, showed no labeling. Our results indicate that rhodopsin and green cone opsin have a similar antigenic determinant, and that two different structural forms of red cone opsin may be present in the turtle retina.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Fotorreceptoras / Pigmentos Retinianos / Segmento Externo de la Célula en Bastón / Tortugas / Anticuerpos Monoclonales Límite: Animals Idioma: En Revista: Exp Eye Res Año: 1990 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Fotorreceptoras / Pigmentos Retinianos / Segmento Externo de la Célula en Bastón / Tortugas / Anticuerpos Monoclonales Límite: Animals Idioma: En Revista: Exp Eye Res Año: 1990 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido