Evidence of neuropeptide bivalency in the interaction of substance P with cerebroside sulfate.

Appu Rao, A G; Cann, J R; Stewart, J M; Winzor, D J

Appu Rao, A G; Cann, J R; Stewart, J M; Winzor, D J.

Afiliación

Appu Rao AG; Department of Biochemistry/Biophysics/Genetics, University of Colorado Health Sciences Center, Denver.

Int J Pept Protein Res ; 35(3): 258-62, 1990 Mar.

Article en En | MEDLINE | ID: mdl-1693915

RESUMEN

A precipitin effect has been observed with mixtures of cerebroside sulfate and the neuropeptide substnace P. This phenomenon is attributed to multivalency of the lipid due to its existence in micellar form, and to bivalency of substance P. One of those neuropeptide sites is almost certainly the basic residue(s) located at the N-terminal of substance P, whereas the hydrophobic residues at the C-terminus are suggested as candidates for the other site on the basis of turbidimetric, circular dichroic, and fluorometric studies. An intrinsic association constant of 3.6 x 10(4)M-1 has been obtained from the cerebroside sulfate concentration associated with maximal turbidity of mixtures containing a fixed concentration of the neuropeptide.

Asunto(s)

Cerebrósidos/metabolismo; Sustancia P/metabolismo; Sulfatos/metabolismo; Sitios de Unión; Fenómenos Químicos; Química Física; Dicroismo Circular; Electroquímica; Micelas; Nefelometría y Turbidimetría; Solubilidad; Soluciones; Espectrometría de Fluorescencia

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfatos / Sustancia P / Cerebrósidos Idioma: En Revista: Int J Pept Protein Res Año: 1990 Tipo del documento: Article Pais de publicación: Dinamarca

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