Inactivation of vesicular stomatitis virus through inhibition of membrane fusion by chemical modification of the viral glycoprotein.
Antiviral Res
; 73(1): 31-9, 2007 Jan.
Article
en En
| MEDLINE
| ID: mdl-16934341
Membrane fusion is an essential step in the entry of enveloped viruses into their host cells triggered by conformational changes in viral glycoproteins. We have demonstrated previously that modification of vesicular stomatitis virus (VSV) with diethylpyrocarbonate (DEPC) abolished conformational changes on VSV glycoprotein and the fusion reaction catalyzed by the virus. In the present study, we evaluated whether treatment with DEPC was able to inactivate the virus. Infectivity and viral replication were abolished by viral treatment with 0.5mM DEPC. Mortality profile and inflammatory response in the central nervous system indicated that G protein modification with DEPC eliminates the ability of the virus to cause disease. In addition, DEPC treatment did not alter the conformational integrity of surface proteins of inactivated VSV as demonstrated by transmission electron microscopy and competitive ELISA. Taken together, our results suggest a potential use of histidine (His) modification to the development of a new process of viral inactivation based on fusion inhibition.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glicoproteínas de Membrana
/
Proteínas del Envoltorio Viral
/
Virus de la Estomatitis Vesicular Indiana
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Inactivación de Virus
/
Dietil Pirocarbonato
/
Fusión de Membrana
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Antiviral Res
Año:
2007
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Países Bajos