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GABAergic structures in the ventral part of the oral pontine reticular nucleus: An ultrastructural immunogold analysis.
de la Roza, C; Reinoso-Suárez, F.
Afiliación
  • de la Roza C; Departamento de Anatomía, Fisiología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo s.n., 28029 Madrid, Spain. carmen.roza@uam.es
Neuroscience ; 142(4): 1183-93, 2006 Nov 03.
Article en En | MEDLINE | ID: mdl-16916586
GABA mediates inhibitory effects in neurons of the ventral part of the oral pontine reticular nucleus (vRPO). Evidence increasingly suggests that GABA plays an important role in the modulation of rapid eye movement (REM) sleep generation in the cat vRPO. Here, we investigate the anatomical substrate of this modulation using GABA immunocytochemistry. Immunoperoxidase labeling revealed a few small GABA-immunoreactive cell bodies scattered throughout the vRPO. The numerical densities of all vRPO synapses and the GABA-immunoreactive synapses were estimated, at the electron microscopical level, by using a combination of the physical disector and the post-embedding immunogold techniques. We estimated that 30% of all vRPO synaptic terminals were immunoreactive to GABA. Our findings support the hypothesis that vRPO neuron activity is significantly controlled by inhibitory GABAergic terminals that directly target somata and the different parts of the dendritic tree, including distal regions. GABAergic input could inhibit vRPO REM sleep-inducing neurons during other states of the sleep-wakefulness cycle such as wakefulness or non-REM sleep.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Formación Reticular / Puente / Terminales Presinápticos / Ácido gamma-Aminobutírico / Inhibición Neural / Vías Nerviosas Límite: Animals Idioma: En Revista: Neuroscience Año: 2006 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Formación Reticular / Puente / Terminales Presinápticos / Ácido gamma-Aminobutírico / Inhibición Neural / Vías Nerviosas Límite: Animals Idioma: En Revista: Neuroscience Año: 2006 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos