Mapping of a dominant immunogenic region of synaptophysin, a major membrane protein of synaptic vesicles.
FEBS Lett
; 261(2): 358-60, 1990 Feb 26.
Article
en En
| MEDLINE
| ID: mdl-1690151
Synaptophysin is a major integral membrane protein of synaptic vesicles. Its transmembrane topology deduced from the cDNA sequence predicts 4 transmembrane regions and a carboxy-terminal cytoplasmic tail containing a characteristic pentapeptide repeat structure. The monoclonal antibody (mAb), SY38, binds to a cytoplasmic domain of synaptophysin. By using fusion proteins corresponding to truncated forms of the cytoplasmic tail, its epitope was located to a flexible segment in the center of the repeat structure. Four other mAbs (c7.1, c7.2, c7.3, c7.4) share the same epitope, which thus emerges as the major immunogenic region of this membrane protein.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vesículas Sinápticas
/
Proteínas de la Membrana
/
Epítopos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Reino Unido