Expression and polymorphism of a Trypanosoma cruzi gene encoding a cytoplasmic repetitive antigen.
Exp Parasitol
; 70(3): 247-54, 1990 Apr.
Article
en En
| MEDLINE
| ID: mdl-1690143
The study of the expression of a Trypanosoma cruzi gene encoding a cytoplasmic repetitive antigen (CRA) during the metacyclogenesis process shows that this gene is not expressed in metacyclic trypomastigote forms of the parasite. However, a slight increase in CRA expression was observed following the nutritional stress of epimastigotes which precedes T. cruzi metacyclogenesis in vitro. The comparison of the expression of CRA in different T. cruzi strains shows that this gene is highly polymorphic: some strains display one and others display two polypeptides reacting with a CRA antiserum. The comparison of T. cruzi G-49 strain and Dm 28c clone shows that they display rather different Northern and Southern blot profiles when probed with a clone corresponding to the repetitive region of the CRA gene. A similar polymorphism was also observed for the gene encoding a flagellar repetitive antigen, suggesting that gene polymorphism might be a common feature of many T. cruzi genes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polimorfismo Genético
/
Trypanosoma cruzi
/
Expresión Génica
/
Antígenos de Protozoos
Límite:
Animals
Idioma:
En
Revista:
Exp Parasitol
Año:
1990
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos