Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years.
Clin Microbiol Infect
; 12(8): 815-8, 2006 Aug.
Article
en En
| MEDLINE
| ID: mdl-16842583
This study examined the ability of PCR to amplify Leishmania DNA, stored on Giemsa-stained slides, from American cutaneous leishmaniasis (ACL) patients. In total, 475 slides stored for up to 36 years were obtained from an outpatient clinic in a Brazilian ACL-endemic region, and Leishmania DNA was amplified from 395 (83.2%) of the DNA samples using primers specific for the minicircle kinetoplast DNA. Restriction fragment length polymorphism analysis of these amplicons demonstrated that Leishmania (Viannia) braziliensis was the only species present in these samples. The results demonstrated that archived Giemsa-stained slides can provide a Leishmania DNA source for performing clinical and epidemiological studies of leishmaniasis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Leishmania braziliensis
/
Reacción en Cadena de la Polimerasa
/
Leishmaniasis Cutánea
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Clin Microbiol Infect
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Reino Unido