Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells.
J Neurochem
; 98(2): 459-70, 2006 Jul.
Article
en En
| MEDLINE
| ID: mdl-16805839
To investigate the effects of suppressors of cytokine signaling 3 (SOCS3) on neural stem cell fate, stem cells were infected with an adenoviral vector expressing SOCS3. Three days later, western blot analysis and immunocytochemical analysis revealed that the protein level of MAP2 and the number of MAP2-positive cells were significantly increased in SOCS3-transfected cells, whereas the protein level of GFAP and the number of GFAP-positive cells were significantly decreased. Furthermore, promoter assay revealed a significant reduction in the transcriptional level of signal transducer and activator of transcription 3 (Stat3) in the transfected cells. In addition, the mRNA levels of Notch family member (notch1) and inhibitory basic helix-loop-helix (bHLH) factors (hes5 and id3) were significantly up-regulated 1 day after overexpression of SOCS3. Three days after transfection, the mRNA level of hes5 was significantly decreased, whereas that of notch1 was still up-regulated. Moreover, all of SOCS3-positive cells expressed Nestin protein but did not express MAP2 or GFAP proteins. These data indicate that overexpression of SOCS3 induced neurogenesis and inhibited astrogliogenesis in neural stem cells. Our data also show that SOCS3 promoted maintenance of neural stem cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Astrocitos
/
Proteínas Supresoras de la Señalización de Citocinas
/
Neuronas
Límite:
Humans
Idioma:
En
Revista:
J Neurochem
Año:
2006
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido