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Microarray analysis of BeWo and JEG3 trophoblast cell lines: identification of differentially expressed transcripts.
Burleigh, D W; Kendziorski, C M; Choi, Y J; Grindle, K M; Grendell, R L; Magness, R R; Golos, T G.
Afiliación
  • Burleigh DW; Wisconsin National Primate Research Center, University of Wisconsin Medical School, 1220 Capitol Court, Madison, WI 53715-1299, USA.
Placenta ; 28(5-6): 383-9, 2007.
Article en En | MEDLINE | ID: mdl-16797695
Trophoblast cell lines are important research tools used as a surrogate for primary trophoblast cells in the study of placental function. Because the cellular origins of transformed trophoblasts are likely to be diverse, it would be of value to understand the unique and shared phenotypes of the cells on a global scale. We have compared two widely used cell lines, BeWo and JEG3, by microarray analysis in order to identify differentially expressed genes. Results indicated that approximately 2700 genes were differentially expressed between the cell lines, with principal differences observed in the biological processes of response to stress, cell adhesion, signal transduction, and protein and nucleobase metabolisms. These data suggest that BeWo and JEG3 cell lines, and perhaps other trophoblast cell lines, are sufficiently dissimilar from each other such that they will be differentially suited for specific experimental paradigms.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trofoblastos / Regulación del Desarrollo de la Expresión Génica / Análisis de Secuencia por Matrices de Oligonucleótidos Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Placenta Año: 2007 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trofoblastos / Regulación del Desarrollo de la Expresión Génica / Análisis de Secuencia por Matrices de Oligonucleótidos Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Placenta Año: 2007 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos