Oxidative DNA glycosylases: recipes from cloning to characterization.

Bandaru, Viswanath; Blaisdell, Jeffrey O; Wallace, Susan S

Bandaru, Viswanath; Blaisdell, Jeffrey O; Wallace, Susan S.

Afiliación

Bandaru V; Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington, USA.

Methods Enzymol ; 408: 15-33, 2006.

Article en En | MEDLINE | ID: mdl-16793360

RESUMEN

As new organisms are being sequenced on a daily basis, new DNA glycosylases that recognize DNA damage can be easily identified in an effort to understand both their phylogenetics and substrate specificities. As a practical matter, existing bacterial and human homologs need to be readily available as laboratory reagents in order to compare the activities of the novel enzymes to existing enzymes. This chapter attempts to provide a primer for cloning, expression, and assay procedures for bacterial and human DNA glycosylases that recognize oxidative DNA damages. These methodologies can be translated readily to novel DNA glycosylases or to DNA glycosylases that recognize other types of DNA damages.

Asunto(s)

Clonación Molecular; ADN Glicosilasas/genética; ADN Glicosilasas/metabolismo; Daño del ADN; Humanos; Oxidación-Reducción

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clonación Molecular / ADN Glicosilasas Límite: Humans Idioma: En Revista: Methods Enzymol Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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