In vivo and in vitro immunosuppression by boar seminal vesicle fluid fraction.
Int J Fertil
; 36(3): 183-8, 1991.
Article
en En
| MEDLINE
| ID: mdl-1678375
Boar seminal vesicle fluid inhibits blast transformation of porcine lymphocytes. Boar seminal vesicle fluid was precipitated in 8% ethanol and the dissolved precipitate separated into two peaks upon chromatography on a Sephacryl S-200 column. The inhibitory activity was found predominantly in the second peak. This peak was rechromatographed on the Sephacryl S-200 column, and the fractions with inhibitory activity were pooled and passed down a Sephadex G-75 column. This chromatography run separated the accompanying protease inhibitor from the immunosuppressive fraction. The molecular mass, estimated by chromatography on the Sephadex G-75 column, was 25,000-27,000 for the immunosuppressive factor and 7,500 for the protease inhibitor. Activity for the immunosuppressive fraction was also demonstrated in vivo.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Semen
/
Tolerancia Inmunológica
Límite:
Animals
Idioma:
En
Revista:
Int J Fertil
Año:
1991
Tipo del documento:
Article
Pais de publicación:
Estados Unidos