Development of an in vitro rat intestine segmental perfusion model to investigate permeability and predict oral fraction absorbed.
Pharm Res
; 23(7): 1543-53, 2006 Jul.
Article
en En
| MEDLINE
| ID: mdl-16779709
PURPOSE: The aims of the study are to develop and evaluate an in vitro rat intestine segmental perfusion model for the prediction of the oral fraction absorbed of compounds and to assess the ability of the model to study intestinal metabolism. METHODS: The system consisted of a perfusion cell with a rat intestinal segment and three perfusion circulations (donor, receiver, and rinsing circulation). Lucifer yellow (LY) was applied as internal standard together with test compounds in the donor circulation. To validate the model, the permeability of eight noncongeneric passively absorbed drugs was determined. Intestinal N-demethylation of verapamil into norverapamil was followed in the donor and receiver circulations by high-performance liquid chromatography analysis. RESULTS: The in vitro model allowed ranking of the tested compounds according to their in vivo absorption potential. The Spearman's correlation coefficient between the oral fraction absorbed in humans and the ratio of permeation coefficient of test compound to the permeation coefficient of LY within the same experiment was 0.98 (P < 0.01). Moreover, intestinal N-demethylation of verapamil, its permeation, and the permeation of its metabolite norverapamil could be assessed in parallel. CONCLUSIONS: Up to six permeation kinetics can be obtained per rat, and the method has shown to be a valuable tool to estimate human oral absorption.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Perfusión
/
Preparaciones Farmacéuticas
/
Absorción Intestinal
/
Yeyuno
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Pharm Res
Año:
2006
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Estados Unidos