Mass spectrometric profiling - a diagnostic tool in fish?
Mar Environ Res
; 62 Suppl: S105-8, 2006 Jul.
Article
en En
| MEDLINE
| ID: mdl-16764920
The development of rapid and sensitive diagnostic tools to assess the effect of stressors on organisms is a principal objective of environmental proteomics. This study is focused on evaluating the potential of using surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF MS) to assess stress in Atlantic salmon (Salmo salar). Plasma and mucus samples were taken from fish that had previously been maintained in a range of high density conditions, together with control fish maintained under low density conditions. Samples were collected during the post-density stress period for protein profile analysis. The mass spectra were analysed to evaluate reproducibility and to search for condition specific changes in protein expression. Multivariate analysis of the peak relative intensity data indicated a segregation of the data into three entities in accordance with the density level fish had been subjected to during the density stress period. This segregation was seen in both plasma and mucus data.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estrés Fisiológico
/
Proteínas
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Salmo salar
/
Análisis por Matrices de Proteínas
/
Enfermedades de los Peces
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mar Environ Res
Asunto de la revista:
BIOLOGIA
/
SAUDE AMBIENTAL
/
TOXICOLOGIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Noruega
Pais de publicación:
Reino Unido