Identification of mutations that disrupt phosphorylation-dependent nuclear export of cyclin D1.

Benzeno, S; Lu, F; Guo, M; Barbash, O; Zhang, F; Herman, J G; Klein, P S; Rustgi, A; Diehl, J A

Benzeno, S; Lu, F; Guo, M; Barbash, O; Zhang, F; Herman, J G; Klein, P S; Rustgi, A; Diehl, J A.

Afiliación

Benzeno S; Department of Cancer Biology, The Leonard and Madlyn Abramson Family Cancer Research Institute and Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA.

Oncogene ; 25(47): 6291-303, 2006 Oct 12.

Article en En | MEDLINE | ID: mdl-16732330

RESUMEN

Although cyclin D1 is overexpressed in a significant number of human cancers, overexpression alone is insufficient to promote tumorigenesis. In vitro studies have revealed that inhibition of cyclin D1 nuclear export unmasks its neoplastic potential. Cyclin D1 nuclear export depends upon phosphorylation of a C-terminal residue, threonine 286, (Thr-286) which in turn promotes association with the nuclear exportin, CRM1. Mutation of Thr-286 to a non-phosphorylatable residue results in a constitutively nuclear cyclin D1 protein with significantly increased oncogenic potential. To determine whether cyclin D1 is subject to mutations that inhibit its nuclear export in human cancer, we have sequenced exon 5 of cyclin D1 in primary esophageal carcinoma samples and in cell lines derived from esophageal cancer. Our work reveals that cyclin D1 is subject to mutations in primary human cancer. The mutations identified specifically disrupt phosphorylation of cyclin D1 at Thr-286, thereby enforcing nuclear accumulation of cyclin D1. Through characterization of these mutants, we also define an acidic residue within the C-terminus of cyclin D1 that is necessary for recognition and phosphorylation of cyclin D1 by glycogen synthase kinase-3 beta. Finally, through construction of compound mutants, we demonstrate that cell transformation by the cancer-derived cyclin D1 alleles correlates with their ability to associate with and activate CDK4. Our data reveal that cyclin D1 is subject to mutations in primary human cancer that specifically disrupt phosphorylation-dependent nuclear export of cyclin D1 and suggest that such mutations contribute to the genesis and progression of neoplastic growth.

Asunto(s)

Carcinoma/metabolismo; Núcleo Celular/metabolismo; Ciclina D1/genética; Ciclinas/genética; Neoplasias Esofágicas/metabolismo; Proteínas de Neoplasias/genética; Procesamiento Proteico-Postraduccional; Alelos; Sustitución de Aminoácidos; Animales; Carcinoma/genética; Carcinoma/patología; Línea Celular; Línea Celular Tumoral/química; Transformación Celular Neoplásica/genética; Ciclina D; Ciclina D1/metabolismo; Quinasa 4 Dependiente de la Ciclina/metabolismo; Ciclinas/metabolismo; Análisis Mutacional de ADN; ADN de Neoplasias/genética; Neoplasias Esofágicas/genética; Neoplasias Esofágicas/patología; Glucógeno Sintasa Quinasa 3/metabolismo; Glucógeno Sintasa Quinasa 3 beta; Humanos; Ratones; Mutación Missense; Células 3T3 NIH; Proteínas de Neoplasias/metabolismo; Fosforilación; Fosfotreonina/metabolismo; Mutación Puntual; Transporte de Proteínas/genética; Proteínas Recombinantes de Fusión/metabolismo; Eliminación de Secuencia; Spodoptera

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Esofágicas / Carcinoma / Núcleo Celular / Procesamiento Proteico-Postraduccional / Ciclinas / Ciclina D1 / Proteínas de Neoplasias Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Oncogene Asunto de la revista: BIOLOGIA MOLECULAR / NEOPLASIAS Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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