Primary CCR5 only using HIV-1 isolates does not accurately represent the in vivo replicating quasi-species.
Virology
; 351(2): 489-96, 2006 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-16712896
Most HIV-1 isolates depend on CCR5 or CXCR4 to infect target cells, and efficient use of other coreceptors is rare. We cloned HIV-1 envelopes from virus at acute infection and found that most use CCR3 efficiently. This result contradicts prevailing data, suggesting that CCR3 usage is rare. We hypothesized that direct isolation into PBMC biases selection of viruses that use CCR5 and not CCR3. We therefore compared coreceptor use of isolates obtained by PBMC coculture with envelopes cloned directly from patient blood samples, which should represent actively replicating species. Viruses derived by cloning generally used CCR3 and CCR5 with equally efficiently. In contrast, we found that viruses isolated by PBMC coculture largely, or exclusively, used CCR5. Regardless of whether CCR3 use contributes to HIV-1 transmission or pathogenesis, our results demonstrate that "primary isolates" generated by PBMC culture are unlikely to accurately represent the in vivo replicating quasi-species.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Replicación Viral
/
VIH-1
/
Receptores CCR5
Límite:
Humans
/
Male
Idioma:
En
Revista:
Virology
Año:
2006
Tipo del documento:
Article
Pais de publicación:
Estados Unidos