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The role of the C-terminal region of olive latent virus 1 coat protein in host systemic infection.
Pantaleo, V; Grieco, F; Di Franco, A; Martelli, G P.
Afiliación
  • Pantaleo V; Istituto di Virologia Vegetale del CNR, Sezione di Bari and Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi, Bari, Italy. v.pantaleo@ba.ivv.cnr.it
Arch Virol ; 151(10): 1973-83, 2006 Oct.
Article en En | MEDLINE | ID: mdl-16699830
A full-length cDNA clone of olive latent virus 1 (OLV-1), a member of the genus Necrovirus, family Tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. A mutant clone, denoted Delta3297, was obtained by deleting the nucleotide in position 3297, thus inducing a frameshift and replacing the last 49 amino acids of the viral coat protein (CP) by a shorter sequence of 39 amino acids. This mutant was viable, stable, able to synthesize a smaller CP, and able to give rise to the formation of apparently intact virus particles. Cell-to-cell movement of Delta3297 in Nicotiana benthamiana leaves was not affected, but, contrary to wild type OLV-1, it failed to spread systemically. These results indicate that virion formation is necessary but not sufficient for long-distance movement for OLV-1 and highlights the role of the CP carboxy-terminal domain in systemic infection.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Infecciones por Virus ARN / Tombusviridae / Proteínas de la Cápside Idioma: En Revista: Arch Virol Año: 2006 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Austria
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Infecciones por Virus ARN / Tombusviridae / Proteínas de la Cápside Idioma: En Revista: Arch Virol Año: 2006 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Austria