Identification of the infectious laryngotracheitis virus glycoprotein gB gene by the polymerase chain reaction.
Virus Genes
; 5(4): 335-47, 1991 Oct.
Article
en En
| MEDLINE
| ID: mdl-1665614
The infectious laryngotracheitis virus (ILTV) homologue of the herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene was identified by PCR amplification of genomic ILTV DNA. A 488-bp amplified DNA fragment was used to identify and clone two adjacent PstI fragments from genomic ILTV DNA. Sequence analysis of the region surrounding the amplified fragment identified a 2619-bp open reading frame that has 39% homology with both the nucleotide and amino-acid sequences of the HSV-1 gB gene. Northern blot analysis using a portion of the open reading frame as a probe identified a 2.7-kb RNA transcript in ILTV-infected chicken embryo liver cells. Analysis of the predicted amino acid sequence of the ILTV protein indicated that it shares structural features with the gB glycoproteins of other herpesviruses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas del Envoltorio Viral
/
Simplexvirus
/
Herpesvirus Gallináceo 1
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Virus Genes
Asunto de la revista:
BIOLOGIA MOLECULAR
/
VIROLOGIA
Año:
1991
Tipo del documento:
Article
Pais de publicación:
Estados Unidos