[Production purification and characterization of polyantibodies against microcystin-LR].
Wei Sheng Yan Jiu
; 35(1): 76-8, 2006 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-16598941
OBJECTIVE: Production and characterization the polyantibodies against MCYST-LR. METHODS: microcystin-LR (MC-LR) was conjugated to KLH, then immune the rabbit by the routine method. Purify the antiserum by centrifugal and saturated sulfuric ammonium precipitated methods. Count the affinity constant and measure the titers, IC50 Value and the sensitivity. RESULTS: The titers of the antiserum is over 25600. IC50 value is about from 0.1 ng/ml to 1 ng/ml. The across-reactivity ratio to MC-LW and MC-LR is about from 0.1% to 20%.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microcistinas
/
Anticuerpos Antibacterianos
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
Zh
Revista:
Wei Sheng Yan Jiu
Asunto de la revista:
SAUDE PUBLICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China