[Screening for Y chromosomal microdeletions in AZF region with modified multiplex PCR].
Zhonghua Nan Ke Xue
; 12(3): 199-201, 206, 2006 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-16597030
OBJECTIVE: Screening for Y chromosomal microdeletions in azoospermia factor (AZF) region with modified multiplex PCR. METHODS: 160 cases with spermatogenetic failure were recruited in the experimental group, while 90 cases of donors in controls. According to the laboratory guidelines supported by European Academy of Andrology (EAA) and European Molecular Genetics Quality Network (EMQN), Y chromosomal microdeletions in AZFa, b, c regions were screened with multiplex PCR. The primers of sequence targeted sites (STSs) and conditions of PCR were modified. RESULTS: Using modified multiplex PCR, 14 (8.75%) cases with Y chromosomal microdeletions were found in the experimental group, while no case in controls. There were 12 cases in AZFc, 1 case in AZFa + b + c, 1 case in AZFb + c. According to statistics, the difference between two groups was significant (P <0.001). Reaction products could be clearly separated with agarose gel and finished in 1 h. CONCLUSION: Modified multiplex PCR protocols supported by EAA and EMQN proved to be very accurate, sensitive and quick, which could be put into screening practice for Y chromosomal microdeletions in AZF region.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Deleción Cromosómica
/
Cromosomas Humanos Y
Tipo de estudio:
Diagnostic_studies
/
Guideline
/
Screening_studies
Límite:
Adult
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2006
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China