Medroxyprogesterone acetate stimulates cdk inhibitors, p21 and p27, in endometrial carcinoma cells transfected with progesterone receptor-B cDNA.
Eur J Gynaecol Oncol
; 27(1): 33-8, 2006.
Article
en En
| MEDLINE
| ID: mdl-16550965
PURPOSE OF INVESTIGATION: Progestin is reported to suppress the growth of endometrial carcinomas, although its precise mechanism of action is not clear. This study aimed to transfect progesterone receptor-B (PRB) cDNA into endometrial carcinoma cells and investigate the effect of medroxyprogesterone acetate (MPA) on cell growth, and p21 and p27 expression in the transfectant. METHODS: Immunoblotting for p21 and p27 was performed at predetermined times after the administration of MPA. RESULTS: PR expression was maximally induced in Ishikawa cells at 24 hrs after the transfection. At 1 x 10(-6) M, MPA suppressed the growth of the transfectant by 34% on day 6 and stimulated p21 accumulation at 48 to 72 hrs and p27 accumulation at 48 to 96 hrs after its administration. PRB cDNA was effectively transfected and in the transfectant MPA at 1 x 10(-6) M, the dosage suppressing growth, induced p21 and p27expression. CONCLUSION: p21 and p27 may be related to progesterone-induced growth suppression in human endometrial adenocarcinoma.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Tumorales Cultivadas
/
Receptores de Progesterona
/
Acetato de Medroxiprogesterona
/
Inhibidor p21 de las Quinasas Dependientes de la Ciclina
/
Inhibidor p27 de las Quinasas Dependientes de la Ciclina
Tipo de estudio:
Diagnostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Eur J Gynaecol Oncol
Año:
2006
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Singapur