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Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database.
Hill, Janet E; Paccagnella, Ana; Law, Kee; Melito, Pasquale L; Woodward, David L; Price, Lawrence; Leung, Amy H; Ng, Lai-King; Hemmingsen, Sean M; Goh, Swee Han.
Afiliación
  • Hill JE; National Research Council Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada.
  • Paccagnella A; British Columbia Centre for Disease Control, 655W 12th Ave, Vancouver, BC, V5Z 4R4, Canada.
  • Law K; British Columbia Centre for Disease Control, 655W 12th Ave, Vancouver, BC, V5Z 4R4, Canada.
  • Melito PL; Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
  • Woodward DL; Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
  • Price L; Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
  • Ng LK; Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
  • Hemmingsen SM; National Research Council Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada.
  • Goh SH; British Columbia Centre for Disease Control, 655W 12th Ave, Vancouver, BC, V5Z 4R4, Canada.
J Med Microbiol ; 55(Pt 4): 393-399, 2006 Apr.
Article en En | MEDLINE | ID: mdl-16533986
A robust method for the identification of Campylobacter spp. based on direct sequencing of PCR-amplified partial cpn60 sequences and comparison of these to a reference database of cpn60 sequences is reported. A total of 53 Campylobacter isolates, representing 15 species, were identified and distinguished from phenotypically similar Helicobacter and Arcobacter strains. Pairwise cpn60 sequence identities between Campylobacter spp. ranged from 71 to 92 %, with most between 71 and 79 %, making discrimination of these species obvious. The method described overcomes limitations of existing PCR-based methods, which require time-consuming and complex post-amplification steps such as the cloning of amplification products. The results of this study demonstrate the potential for use of the reference chaperonin sequence database, cpnDB, as a tool for identification of bacterial isolates based on cpn60 sequences amplified with universal primers.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Campylobacter / Helicobacter / Chaperoninas / Arcobacter / Bases de Datos Genéticas Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Med Microbiol Año: 2006 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Campylobacter / Helicobacter / Chaperoninas / Arcobacter / Bases de Datos Genéticas Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Med Microbiol Año: 2006 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido