Semidehydroascorbic acid as an intermediate in norepinephrine biosynthesis in chromaffin granules.
J Biol Chem
; 266(20): 12908-14, 1991 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-1649168
We investigated whether semidehydroascorbic acid was an intermediate in norepinephrine synthesis in chromaffin granules and in electron transfer across the chromaffin granule membrane. Semidehydroascorbic acid was measured in intact granules by electron spin resonance. In the presence of intragranular but not extragranular ascorbic acid, semidehydroascorbic acid was formed within granules in direct relationship to dopamine beta-monooxygenase activity. However, semidehydroascorbic acid was not generated when granules were incubated with epinephrine instead of the substrate dopamine, with dopamine beta-monooxygenase inhibitors, without oxygen, and when intragranular ascorbic acid was depleted. Experiments using the impermeant paramagnetic broadening agents [K3 [Cr(C2O4)3].3H2O] and Ni(en)3(NO3)2 provided further evidence that semidehydroascorbic acid was generated only within granules. We also investigated semidehydroascorbic acid formation in the presence of intragranular and extragranular ascorbic acid. Under these conditions, semidehydroascorbic acid was formed on both sides of the granule membrane, and formation was coupled to dopamine beta-monooxygenase activity. These data indicate that dopamine beta-monooxygenase is reduced by single electron transfer from intragranular ascorbic acid, that transmembrane electron transfer occurs by single electron transfer, and that transmembrane electron transfer is directly coupled to formation of intragranular semidehydroascorbic acid via dopamine beta-monooxygenase activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Norepinefrina
/
Gránulos Cromafines
/
Médula Suprarrenal
/
Ácido Deshidroascórbico
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
1991
Tipo del documento:
Article
Pais de publicación:
Estados Unidos