Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor.
Methods Enzymol
; 403: 599-607, 2005.
Article
en En
| MEDLINE
| ID: mdl-16473623
Rab/Ypt GTPases are key regulators of intracellular traffic in eukaryotic cells. One important function of Rab/Ypts is the nucleotide-dependent recruitment of downstream effector molecules onto the membrane of organelles. In budding yeast Ypt6 is required for recycling of membrane proteins from endosomes back to the Golgi. A biochemical approach based on the affinity purification of Ypt6:GTP-interacting proteins from yeast cytosol led to the identification of two conserved Ypt6 effectors, the tetrameric VFT complex and Sgm1. The mammalian homolog of Sgm1, TMF/ARA160, contains a short conserved coiled-coil motif that is sufficient for the binding to the three mammalian orthologs of Ypt6, Rab6A, Rab6A', and Rab6B.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Cromatografía de Afinidad
/
Proteínas de Unión al GTP Monoméricas
/
Proteínas de Unión al GTP rab
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas Adaptadoras Transductoras de Señales
/
Proteínas de Unión al ADN
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Methods Enzymol
Año:
2005
Tipo del documento:
Article
Pais de publicación:
Estados Unidos