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Acute liver damage induced by 2-nitropropane in rats: effect of diphenyl diselenide on antioxidant defenses.
Borges, Lysandro P; Nogueira, Cristina Wayne; Panatieri, Rodrigo B; Rocha, João Batista Teixeira; Zeni, Gilson.
Afiliación
  • Borges LP; Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil.
Chem Biol Interact ; 160(2): 99-107, 2006 Mar 25.
Article en En | MEDLINE | ID: mdl-16445897
The effect of post-treatment with diphenyl diselenide on liver damage induced by 2-nitropropane (2-NP) was examined in male rats. Rats were pre-treated with a single dose of 2-NP (100 mg/kg body weight dissolved in canola oil). Afterward, the animals were post-treated with a dose of diphenyl diselenide (10, 50 or 100 micromol/kg). The parameters that indicate tissue damage such as liver histopathology, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), urea and creatinine were determined. Since the liver damage induced by 2-NP is related to oxidative damage, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT) and ascorbic acid level were also evaluated. Diphenyl diselenide (50 and 100 micromol/kg) effectively restored the increase of ALT and AST activities and urea level when compared to the 2-NP group. At the higher dose, diphenyl diselenide decreased GGT activity. Treatment with diphenyl diselenide, at all doses, effectively ameliorated the increase of hepatic and renal lipid peroxidation when compared to 2-NP group. 2-NP reduced CAT activity and neither alter SOD activity nor ascorbic acid level. This study points out the involvement of CAT activity in 2-NP-induced acute liver damage and suggests that the post-treatment with diphenyl diselenide was effective in restoring the hepatic damage induced by 2-NP.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Propano / Solventes / Derivados del Benceno / Compuestos de Organoselenio / Enfermedad Hepática Inducida por Sustancias y Drogas / Hígado / Nitroparafinas / Antioxidantes Límite: Animals Idioma: En Revista: Chem Biol Interact Año: 2006 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Irlanda
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Propano / Solventes / Derivados del Benceno / Compuestos de Organoselenio / Enfermedad Hepática Inducida por Sustancias y Drogas / Hígado / Nitroparafinas / Antioxidantes Límite: Animals Idioma: En Revista: Chem Biol Interact Año: 2006 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Irlanda