Transcription regulation from a TATA and INR-less promoter: spatial segregation of promoter function.
EMBO J
; 25(4): 811-21, 2006 Feb 22.
Article
en En
| MEDLINE
| ID: mdl-16437157
The mode of regulation of class II genes that lack the known core promoter elements is presently unclear. Here, we studied one such example, the murine CD80 gene. An unusual mechanism was revealed wherein the pre-initiation complex (PIC) first assembled on an upstream, NF-kappaB enhancer element. Notably, this assembly occurred independent of contributions from the core promoter domain, and resulted in a PIC that was competent for transcription initiation. Positioning was subsequently achieved by exploiting the intrinsic architecture of the promoter, by virtue of which the tethered PIC was spatially juxtaposed with the transcription initiation site. Bridging interactions then ensued, through protein-protein contacts, which then enabled the elongation phase of CD80 transcription.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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Regulación de la Expresión Génica
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FN-kappa B
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TATA Box
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Elementos de Facilitación Genéticos
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Antígeno B7-1
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
2006
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Reino Unido