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Identification of a common novel mutation in Saudi patients with argininosuccinic aciduria.
Al-Sayed, M; Alahmed, S; Alsmadi, O; Khalil, H; Rashed, M S; Imtiaz, F; Meyer, B F.
Afiliación
  • Al-Sayed M; Department of Medical Genetics, King Faisal Specialist Hospital & Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia. moeen@kfshrc.edu.sa
J Inherit Metab Dis ; 28(6): 877-83, 2005.
Article en En | MEDLINE | ID: mdl-16435180
We have identified a common novel mutation (Q354X) in the argininosuccinate lyase (ASL) gene in Saudi patients with argininosuccinic aciduria (ASAuria; McKusick 207900). The two index patients were siblings, had a neonatal onset of the disease and were diagnosed based on the clinical presentation and confirmed by analysis of their dried blood spots (DBS) by tandem mass spectrometry (MS/MS). The ASL gene was then analysed by direct sequencing. A further 28 patients with a confirmed diagnosis of ASAuria based on MS/MS of their DBS were tested by sequencing for the presence of the Q354X mutation. This mutation was found in 14 out of the 28 patients (50%) tested. Our work indicates that the Q354X allele is common, may account for 50% of the abnormal ASL genes in the Saudi population, and is likely to be associated with the neonatal form of the disease. We recommend that all patients diagnosed with ASAuria in Saudi Arabia or of Arab origin be tested for this mutation and for Q116X, which has been described previously. In addition, further analysis is needed to identify other underlying disease mutations for ASAuria in the Saudi population.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Predisposición Genética a la Enfermedad / Errores Innatos del Metabolismo de los Aminoácidos Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Child / Child, preschool / Female / Humans / Male / Newborn País/Región como asunto: Asia Idioma: En Revista: J Inherit Metab Dis Año: 2005 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Predisposición Genética a la Enfermedad / Errores Innatos del Metabolismo de los Aminoácidos Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Child / Child, preschool / Female / Humans / Male / Newborn País/Región como asunto: Asia Idioma: En Revista: J Inherit Metab Dis Año: 2005 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos