N-unsubstituted glucosamine residues and other modifications in murein of the obligatory chemolithotroph Thiobacillus neapolitanus.

Baj, J; Grabowska, I; Markiewicz, Z

Baj, J; Grabowska, I; Markiewicz, Z.

Afiliación

Baj J; Institute of Microbiology, University of Warsaw.

Res Microbiol ; 143(1): 47-54, 1992 Jan.

Article en En | MEDLINE | ID: mdl-1641512

RESUMEN

Purified murein from Thiobacillus neapolitanus was poorly digested by lysozyme. It's sensitivity to the enzyme greatly increased after N-acetylation. The murein was found to contain 30 to 35% glucosamine residues lacking N-acetyl groups. It also contained phosphomuramic acid. Further modifications included amidation of diaminopimelic acid in the peptide side chains and a low alanine content. None of these modifications were found in the murein of another sulphur bacterium, Thiobacillus versutus.

Asunto(s)

Peptidoglicano/química; Thiobacillus/metabolismo; Aminoácidos/análisis; Amino Azúcares/análisis; Cromatografía por Intercambio Iónico; Cromatografía en Capa Delgada; Hexosaminas/análisis; Técnicas In Vitro; Ácidos Murámicos/análisis; Muramidasa/metabolismo; Peptidoglicano/aislamiento & purificación

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Thiobacillus / Peptidoglicano Idioma: En Revista: Res Microbiol Asunto de la revista: MICROBIOLOGIA Año: 1992 Tipo del documento: Article Pais de publicación: Francia

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